Enzymes are Proteins which catalyze variety of reaction in the Biological systems. There are many methods used to produce the enzymes among that the biological methods are widely used. In this type of biological method of production, solid state fermentation is applied for the production. In all the types of fermentation processes, the cultures has been prepared using yeast extract and peptone etc. These are added to the culture in terms of nutrients as a carbon and nitrogen sources for the microorganism. The cost of these chemicals are much expensive. So the alternative method has been proposed for the preparation of culture medium using some low cost agricultural byproducts such as defatted cotton seed, defatted soybean, mustard seed etc. The fermentation has to carryout using these type of low cost medium to check the productivity and enzyme activity.