Remedial aspects of plants are owing to existence of secondary metabolites that act as armaments to shield plant body besides trepidation organisms. It was conjecture that Parthenium hystrophours has persuasive antibacterial and antifungal activity so present study was conducted to resolute antibacterial, antifungal activity and preface phytochemical screening of this plant. Subject plant engrossed in three solvents i.e. methanol, ethanol and water after converting in powder form in order to prepared plant organic extracts. Antibacterial activity was determined by using ager well diffusion assay against twelve bacterial strains Escherichia coli 1, Escherichia coli 2, Escherichia coli 3, Neisseria gonorrhoeae, Streptococcus pyogenes, Enterobactor aerogenes, Shigella sonnei, Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus 1, Staphylococcus aureus 2 and Klebsiella pneumonia. A very novel parameter was also applied which involved the effect of time on the doses of extract to inhibit bacterial growth to determine plant crude drug efficacy.