E. coli are commonly found in the intestinal tract of humans and other animal species and constitute a reservoir of resistant genes for potentially pathogenic bacteria. E. coli are one of the important bacterial pathogens responsible for uterine infections in cow. Extended-spectrum beta-lactamases (ESBLs) are a novel group of enzyme, was detected in the mid-1980s among Serratiamarcescens and Klebsiellapneumoniae in Germany which produced by bacteria belongs to Enterobacteriaceae family and confer resistance to an extended-spectrum of cephalosporins besides penicillins and monobactams. From the study it was observed that on average crossbred are most susceptible to reproductive diseases than the local cows and E. coli is one of the etiological factors of uterine infections in cow.A total of 63 E. coli isolates, 45 (71.42%), 43 (68.25%), 44 (69.84%), 40 (63.43%), and 35 (55.55%) isolates were resistant against cefpodoxime, aztreonum, ceftazidime, cefepime, and ceftriaxone, respectively. Furthermore, resistance was also observed totetracycline, ampicillin, gentamicin, norfloxacin, clotrimoxazole and chloramphenicol were 100%, 100%, 84.12%, 95.23%,77.77% and 25.39% respectively.